Human Free BDNF Quantikine ELISA Kit
Human Free BDNF Quantikine ELISA Kit
Human Free BDNF Quantikine ELISA Kit Summary
貨號：DBD00
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (50 uL), Platelet-poor Heparin Plasma (50 uL), Citrate Plasma (50 uL)
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human BDNF
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Human BDNF Immunoassay is a 3.5 hour solid phase ELISA designed to measure human BDNF in cell culture supernates, serum, and plasma. It contains recombinant human BDNF expressed in Sf 21 cells and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant BDNF accurately. Results obtained measuring natural human BDNF showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human BDNF.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Serum, EDTA Plasma, Platelet-poor Heparin Plasma, Citrate Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
476
|
1258
|
2175
|
528
|
1338
|
2287
|
|
Standard Deviation
|
24
|
48.2
|
135.7
|
59.7
|
102.3
|
186
|
|
CV%
|
5
|
3.8
|
6.2
|
11.3
|
7.6
|
8.1
|
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
334
|
825
|
1339
|
358
|
905
|
1517
|
|
Standard Deviation
|
15.6
|
28.3
|
56.5
|
36.8
|
86.8
|
121.6
|
|
CV%
|
4.7
|
3.4
|
4.2
|
10.3
|
9.6
|
8.1
|
Recovery
The recovery of BDNF spiked to three different levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=5)
|
96
|
83-105
|
|
Platelet-poor Citrate Plasma (n=5)
|
99
|
87-120
|
|
Platelet-poor EDTA Plasma (n=5)
|
96
|
89-115
|
|
Platelet-poor Heparin Plasma (n=5)
|
93
|
81-119
|
|
Serum (n=5)
|
100
|
85-116
|
Linearity
To assess the linearity of the assay, five samples containing or spiked with high concentrations of BDNF in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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