Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit
Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit
Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit Summary
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貨號：HSLB00D
Sample Type & Volume Required Per Well
Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Saliva (10 uL)
Assay Range
0.1 - 8 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Saliva)
Specificity
Natural and recombinant human IL-1 beta. This assay also recognizes recombinant rhesus macaque IL-1 beta (aa 117-269).
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine HS Human IL-1 beta Immunoassay is a 4 hour solid phase ELISA designed to measure human IL-1 beta levels in serum, plasma, and saliva. It contains E. coli-expressed recombinant human IL-1 beta and antibodies raised against the recombinant factor and has been shown to accurately quantitate recombinant human IL-1 beta. Results obtained using natural IL-1 beta showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that the Quantikine HS Immunoassay kit can be used to determine relative mass values for natural human IL-1 beta. Reports indicate that ELISA kits calibrated using mature IL-1 beta as a standard will detect, but considerably underestimate, the unprocessed IL-1 beta precursor present in samples. In biological samples other than cell lysates, the precursor form of IL-1 beta is usually not the predominant form of IL-1 beta present and, additionally, is not biologically active. Therefore, results obtained using this kit should provide a useful measure of the levels of active IL-1 beta present in serum, plasma, and saliva.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Serum, EDTA Plasma, Heparin Plasma, Saliva
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Intra-Assay Precision
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Inter-Assay Precision
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Sample
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1
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2
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3
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1
|
2
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3
|
|
n
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20
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20
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20
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20
|
20
|
20
|
|
Mean
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0.315
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1.3
|
2.52
|
0.27
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1.36
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2.6
|
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Standard Deviation
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0.014
|
0.05
|
0.06
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0.029
|
0.101
|
0.163
|
|
CV%
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4.4
|
3.9
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2.4
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10.7
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7.4
|
6.3
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Recovery
The recovery of human IL-1 beta spiked to levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
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Average % Recovery
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Range %
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EDTA Plasma (n=4)
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94
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87-106
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Heparin Plasma (n=4)
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92
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89-99
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Serum (n=4)
|
91
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86-101
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IL-1 beta were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
