熱門關鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
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熱門關鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
全國統(tǒng)一客服熱線：
0755-28715175
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貨號：R1000
規(guī)格：96T
Serum, EDTA Plasma
Cell Culture Supernates
The recovery of rat IL-10 spiked to three levels throughout the range of the assay in various matrices was evaluated.
Rat IL-10 Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Specificity
Natural and recombinant rat IL-10
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Rat IL-10 Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-10 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat IL-10 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant rat IL-10 accurately. Results obtained using natural rat IL-10 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IL-10.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean
138
270
660
132
270
682
Standard Deviation
6.3
9
16.1
13.1
19.7
59.8
CV%
4.6
3.3
2.4
9.9
7.3
8.8
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean
103
218
582
114
236
598
Standard Deviation
5.7
9.5
17.2
10.8
16.4
42.6
CV%
5.5
4.4
3
9.5
6.9
7.1
Recovery
Sample Type
Average % Recovery
Range %
Cell Culture Supernates (n=5)
106
96-118
EDTA Plasma (n=6)
100
88-115
Serum (n=6)
94
82-112
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