熱門關(guān)鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
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熱門關(guān)鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
全國統(tǒng)一客服熱線：
0755-28715175
貨號：DCX900 規(guī)格：96T
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Cell Culture Supernates
Serum, EDTA Plasma, Heparin Plasma
The recovery of MIG spiked to levels throughout the range of the assay in various matrices was evaluated.
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sensitivity
11.3 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human MIG
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human MIG Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human MIG in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MIG and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human MIG showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring MIG.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
40
40
40
Mean
235
700
1358
242
707
1391
Standard Deviation
9.5
25.1
24.3
20.8
50.9
83.6
CV%
4
3.6
1.8
8.6
7.2
6
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
40
40
40
Mean
253
817
1603
245
723
1427
Standard Deviation
9.9
26.6
49.6
12.8
59.4
88.1
CV%
3.9
3.3
3.1
5.2
8.2
6.2
Recovery
Sample Type
Average % Recovery
Range %
Cell Culture Media (n=4)
92
86-98
EDTA Plasma (n=5)
100
95-110
Heparin Plasma (n=5)
109
101-114
Serum (n=5)
108
101-113
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