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Human/Canine/Porcine Insulin Quantikine ELISA Kit
Human/Canine/Porcine Insulin Quantikine ELISA Kit

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Human/Canine/Porcine Insulin Quantikine ELISA Kit

詳情說明

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Human/Canine/Porcine Insulin Quantikine ELISA Kit Summary

貨號：DINS00

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)

Sensitivity

2.15 pmol/L

Assay Range

15.6 - 500 pmol/L (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Recombinant human Insulin and natural human, canine, and porcine Insulin. This assay cross-reacts 0.25% with Human Proinsulin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human/Canine/Porcine Insulin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure Insulin in cell culture supernates, serum, and plasma. It contains S. cerevisiae-expressed recombinant human Insulin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural Insulin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Insulin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	66.8	158	299	62.2	148	288
Standard Deviation	2.58	5.89	11.9	4.6	10.2	21.5
CV%	3.9	3.7	4	7.4	6.9	7.5

Recovery

The recovery of Insulin spiked to levels throughout the range of the assay in various matrices was evaluated

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	95	85-108
EDTA Plasma (n=4)	95	86-102
Heparin Plasma (n=4)	92	85-105
Serum (n=4)	96	87-103

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Insulin were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.