熱門(mén)關(guān)鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
全國(guó)統(tǒng)一客服熱線(xiàn)：
0755-28715175
熱門(mén)關(guān)鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
全國(guó)統(tǒng)一客服熱線(xiàn)：
0755-28715175
貨號(hào)：DCX110 規(guī)格：96T
深圳子科生物優(yōu)勢(shì)代理美國(guó)R&DELISA系列產(chǎn)品試劑盒，價(jià)格優(yōu)勢(shì)，貨期短，歡迎來(lái)電咨詢(xún)0755-33164177！
Serum, EDTA Plasma, Heparin Plasma
Cell Culture Supernates
The recovery of I-TAC spiked to levels throughout the range of the assay in various matrices was evaluated.
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sensitivity
39.7 pg/mL
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human I-TAC
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human I-TAC Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human I-TAC in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human I-TAC and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human I-TAC showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human I-TAC.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
40
40
40
Mean
403
1321
2481
437
1403
2644
Standard Deviation
19
62.5
147.3
35.1
107
190
CV%
4.7
4.7
5.9
8
7.6
7.2
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
40
40
40
Mean
353
1179
2191
409
1290
2401
Standard Deviation
18
40.6
102.9
35.5
93
179
CV%
5.1
3.4
4.7
8.7
7.2
7.5
Recovery
Sample Type
Average % Recovery
Range %
Cell Culture Media (n=4)
100
91-111
EDTA Plasma (n=5)
98
85-112
Heparin Plasma (n=5)
98
90-106
Serum (n=5)
106
93-117
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of I-TAC were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
0755-28715175/33164177
粵公網(wǎng)安備 44030902000304號(hào)
