Human Cytochrome c Quantikine ELISA Kit
Human Cytochrome c Quantikine ELISA Kit
Human Cytochrome c Quantikine ELISA Kit Summary
Catalog # Availability Size / Price
DCTC0 1 Kit / Please Inquire
SCTC0 1 Pack (6 Plates) / Please Inquire
PDCTC0 1 Pack (50 Plates) / Please Inquire
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Lysates (20 uL), Subcellular Fractions (100 uL)
Assay Range
0.6 - 20 ng/mL (Cell Lysates, Subcellular Fractions)
Specificity
Natural human Cytochrome c
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with other substances.
Product Summary
The Quantikine Human Cytochrome c Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human cytochrome c in cell lysates.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Lysates, Subcellular Fractions
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
3.64
|
7.31
|
10.7
|
3.54
|
6.74
|
10.2
|
|
Standard Deviation
|
0.15
|
0.3
|
0.42
|
0.18
|
0.34
|
0.51
|
|
CV%
|
4.1
|
4.1
|
3.9
|
5.1
|
5
|
5
|
Linearity
To assess the linearity of the assay, six cell lysates containing high concentrations of Cytochrome c were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
