Human Galectin-9 Quantikine ELISA Kit
Human Galectin-9 Quantikine ELISA Kit
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Human Galectin-9 Quantikine ELISA Kit Summary
貨號：DGAL90
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Saliva (100 uL), Urine (10 uL), Human Milk (10 uL)
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Specificity
Natural and recombinant human Galectin-9.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Galectin-9 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Galectin-9 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains E. coli-expressed recombinant human Galectin-9 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Galectin-9 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Galectin-9.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Saliva, Human Milk
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
1.34
|
3.58
|
6.13
|
1.44
|
3.43
|
6.05
|
|
Standard Deviation
|
0.05
|
0.12
|
0.22
|
0.08
|
0.18
|
0.32
|
|
CV%
|
3.7
|
3.4
|
3.6
|
5.6
|
5.2
|
5.3
|
Serum, EDTA Plasma, Heparin Plasma, Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
1.44
|
3.77
|
6.29
|
1.4
|
3.28
|
5.84
|
|
Standard Deviation
|
0.06
|
0.07
|
0.08
|
0.11
|
0.2
|
0.3
|
|
CV%
|
4.2
|
1.9
|
1.3
|
7.9
|
6.1
|
5.1
|
Recovery
The recovery of human Galectin-9 spiked to levels throughout the range of the assay was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
96
|
84-104
|
|
EDTA Plasma (n=4)
|
103
|
85-117
|
|
Heparin Plasma (n=4)
|
104
|
89-117
|
|
Serum (n=4)
|
106
|
86-117
|
Linearity
To assess the linearity of the assay, samples containing high concentrations of human Galectin-9 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
