Human Haptoglobin Quantikine ELISA Kit
Human Haptoglobin Quantikine ELISA Kit
Human Haptoglobin Quantikine ELISA Kit Summary
貨號：DHAPG0
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Serum-free Cell Culture Supernates (20 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (20 uL)
Assay Range
3.1 - 200 ng/mL (Serum-free Cell Culture Supern, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Specificity
Natural human haptoglobin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Haptoglobin Immunoassay is a 4.5 hour solid phase ELISA designed to measure haptoglobin in serum-free cell culture supernates, serum, plasma, saliva, and urine
Precision
Intra-Assay Precision (Precision within an assay) Three samples of a known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays
to determine inter-assay precision. Assays were performed by at least
three technicians using two lots of kit components
Serum-free Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
18.7
|
58.3
|
116
|
18.8
|
57.8
|
113
|
|
Standard Deviation
|
0.69
|
1.42
|
3.66
|
1.4
|
3.47
|
6.3
|
|
CV%
|
3.7
|
2.4
|
3.2
|
7.4
|
6
|
5.6
|
Recovery
The recovery of human haptoglobin spiked to levels throughout the range of the assay was evaluated.
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Sample Type
|
Average % Recovery
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Range %
|
|
Serum-free Cell Culture Media (n=4)
|
101
|
94-107
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human haptoglobin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
