Mouse Total MMP-9 Quantikine ELISA Kit Summary
Mouse Total MMP-9 Quantikine ELISA Kit Summary
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Mouse Total MMP-9 Quantikine ELISA Kit Summary
貨號(hào)：MMPT90 規(guī)格：96T
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), Heparin Plasma (10 uL)
Assay Range
0.1 - 5 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma)
Specificity
Natural and recombinant mouse MMP-9 (pro-, active, and TIMP-complexed)
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse MMP-9 (total) Immunoassay is a 4.5 hour solid-phase ELISA designed to measure total mouse MMP-9 (pro-, active, and TIMP-complexed MMP-9) in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse MMP-9 and antibodies raised against the recombinant factor. Natural mouse MMP-9 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative levels of natural mouse MMP-9.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
0.239
|
0.731
|
1.71
|
0.211
|
0.6
|
1.61
|
|
Standard Deviation
|
0.024
|
0.037
|
0.097
|
0.02
|
0.049
|
0.125
|
|
CV%
|
8.4
|
5.1
|
5.7
|
9.6
|
8.1
|
7.8
|
Recovery
The recovery of mouse MMP-9 spiked to 3 levels throughout the range of the assay was evaluated.
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Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Supernates (n=4)
|
94
|
86-108
|
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse MMP-9 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
