熱門(mén)關(guān)鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
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熱門(mén)關(guān)鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
全國(guó)統(tǒng)一客服熱線：
0755-28715175
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貨號(hào)：DHD00C 規(guī)格：96T
Cell Culture Supernates
Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma
The recovery of PDGF-AB spiked to levels throughout the range of the assay in various matrices was evaluated.
Human PDGF-AB Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (10 uL), Platelet-poor EDTA Plasma (100 uL), Platelet-poor Heparin Plasma (100 uL)
Sensitivity
3.83 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)
Specificity
Natural and recombinant human PDGF-AB
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine PDGF-AB Immunoassay is a 4.5 hour solid phase ELISA designed to measure human PDGF-AB in cell culture supernates, serum, and platelet-poor plasma. It contains E. coli-expressed recombinant human PDGF-AB and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PDGF-AB showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PDGF-AB.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean
92.7
312
656
102
343
701
Standard Deviation
8.28
9.73
15.2
10.9
29.9
52.3
CV%
8.9
3.1
2.3
10.7
8.7
7.5
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean
107
350
726
119
362
723
Standard Deviation
6.95
10.9
63.1
10.7
20.9
54.5
CV%
6.5
3.1
8.7
9
5.8
7.5
Recovery
Sample Type
Average % Recovery
Range %
Cell Culture Media (n=4)
105
93-119
Platelet-poor EDTA Plasma (n=4)
104
78-117
Platelet-poor Heparin Plasma (n=4)
105
86-121
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of PDGF-AB were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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